課程概述 |
基因科技學
蔡懷楨
內容:
1.Plasmid and bacteriophages
2.Purification of DNA
3.Manipulation of purified DNA
4.Introduction of DNA into living cells
5.Cloning vectors for E.coli and Eukaryotes
6.How to obtain a clone of a specific gene
7.Polymerase chain reaction
8.Studying gene location and genome structure
9.Studying gene expression and function
10.Studying genomes
11.Production of proteins from cloned genes
12.Gene cloning in medicine and agriculture
13.Transgenic animals and plants
14.Special topics : microarray, stem cells
教科書:T. A. Brown, 2001, Gene Cloning (4th edition)為主
成績評量:期中考或(及)期末考;繳交學期報告為optional.
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